Composite

Part:BBa_K1321295:Design

Designed by: Michael Florea   Group: iGEM14_Imperial   (2014-10-20)


CBDclos fused to sfGFP driven by LacI in pSEVA331-Bb


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4132
    Illegal suffix found in sequence at 1261
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4132
    Illegal SpeI site found at 1262
    Illegal PstI site found at 1276
    Illegal NotI site found at 1269
    Illegal NotI site found at 4138
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4132
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4132
    Illegal suffix found in sequence at 1262
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4132
    Illegal XbaI site found at 4147
    Illegal SpeI site found at 1262
    Illegal PstI site found at 1276
    Illegal NgoMIV site found at 230
    Illegal NgoMIV site found at 2451
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 551


Design Notes

BBa_K1321295 was created by restricting BBa_K1321360 and BBa_K1321300 with PstI and XbaI, gel purifying the resulting fragments, ligating using T4 DNA ligase and transforming the ligase products into chemically competent DH10B cells

Source

Parts BBa_K1321360 and BBa_K1321300

References